Rail, Maritime and Transport (RMT) union members will walk out in dispute over the new shift pattern.
The union said their members were being asked to work both night shifts and day shifts, as dedicated night workers were laid off.
24-hour strike on Night Tube Line – Central, jubilee Northern, Piccadilly and Victoria – starting at 4.30am on Friday, more walkouts are planned in the coming weeks.
to transport London Warned of disruption of services and advised people to check before travelling.
RMT Secretary General Mick Lynch said: “This strike is about the breaking of the popular and family-friendly agreements that helped make the original Night Tube so successful.
“Instead the company wants to cut costs and pile all the drivers into a pool where they can be kicked from pillar to post at the behest of management.”
He said the union had made several attempts to resolve the dispute with the London Underground.
He continued: “This strike action, and its dire consequences for Christmas, could have been avoided if Tube management had not implemented dedicated Night Tube workers and fully workable arrangements to cut down on the workforce and costs.” did.”
Nick Dent, director of London Underground customer operations, said: “RMT’s planned strike action is unnecessary and will threaten London’s recovery from the pandemic, without job losses and greater flexibility and job certainty for drivers.
“While every other union has agreed to these changes and our employees have been taking advantage of the changes since August, we stand ready to work with RMT and review the changes once Night Tube services return. “
Night Tube has been suspended due to the pandemic and is scheduled to resume on Friday.